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dmem high glucose medium  (Danaher Inc)
96
Danaher Inc dmem high glucose medium
Dmem High Glucose Medium, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem high glucose medium/product/Danaher Inc
Average 96 stars, based on 1 article reviews
dmem high glucose medium - by Bioz Stars, 2026-05
96/100 stars
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high glucose dulbecco s modified eagle s medium dmem  (Danaher Inc)
96
Danaher Inc high glucose dulbecco s modified eagle s medium dmem
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
High Glucose Dulbecco S Modified Eagle S Medium Dmem, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high glucose dulbecco s modified eagle s medium dmem/product/Danaher Inc
Average 96 stars, based on 1 article reviews
high glucose dulbecco s modified eagle s medium dmem - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

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FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in DMEM and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.

Journal: Journal of medical virology

Article Title: 25-hydroxycholesterol inhibits human papillomavirus infection in cervical epithelial cells by perturbing cytoskeletal remodeling.

doi: 10.1002/jmv.28834

Figure Lengend Snippet: FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in DMEM and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.

Article Snippet: Cells were cultured in high‐ glucose Dulbecco's modified Eagle's medium (DMEM) (HyClone) supplemented with 10% fetal bovine serum (FBS) (Yeasen), and incubated in a humidified environment containing 5% CO2 at 37°C.

Techniques: Infection, Membrane, Western Blot, Control, Incubation, Inhibition, Phospho-proteomics